ARTP(Atmospheric Room Temperature Plasma)是一种 全新的射频放电技术,该技术使用惰性气体放电,能够在常压室温条件下产生大量高能量的活性粒子;研究表明,活性粒子可以有效的用于细胞的遗传物质并导致DNA结构 损伤,进而利用细胞自身高容错率的修复机制,产生大量的突变位点,最终获得大容量的基因突变库。 These mutants was more than that of the original strain, increased by 28.13 % , 31.17 % and 43.79 %, respectively. Moreover, N-acetyl-L-glutamate synthase activity of these mutants was also increased. Preparative Biochemistry and Biotechnology,2015(E34) |